Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Hdac2

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
B cells
cell type
Primary B cells
tissue
Freshly isolated from spleen
strain
C57BL/6
chip antibody
HDAC2 (GeneTex, GTX109642)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Soluble chromatin was prepared from sonicated nuclei and the sobule chromatin was immunoprecipitated with antibodies. Libraries were prepared with NEBnext Ultra II DNA library prep kit or ThruPLEX DNA-Seq Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
18226850
Reads aligned (%)
95.7
Duplicates removed (%)
29.3
Number of peaks
11568 (qval < 1E-05)

mm9

Number of total reads
18226850
Reads aligned (%)
95.4
Duplicates removed (%)
29.4
Number of peaks
11562 (qval < 1E-05)

Base call quality data from DBCLS SRA